The film 'Once Upon a Time in America' does not feature any identifiable LGBTQ+ characters or themes within its narrative. Consequently, there is no portrayal to evaluate, resulting in an N/A rating for its depiction of LGBTQ+ elements.

The film 'Once Upon a Time in America' does not feature any explicit or implicit transsexual characters or themes. Its narrative focuses on the lives of Jewish-American gangsters, exploring themes of friendship, betrayal, and the American dream without engaging with transgender identity.

The movie does not contain any action or adventure elements.

The film is an adaptation of Harry Grey's novel "The Hoods." All major and minor characters maintain the same gender as established in the source material, with no instances of a character being portrayed on screen as a different gender than their canonical depiction.

The film's characters, primarily Jewish-American, are consistently portrayed by white actors, aligning with the source material and historical context. Shifts in specific ethnicity (e.g., Italian-American actors playing Jewish characters) do not constitute a race swap as the broader racial category remains unchanged.