Peter Rabbit 2: The Runaway does not feature any identifiable LGBTQ+ characters or themes. The narrative centers on the adventures of Peter Rabbit and his animal friends, along with the human characters Bea and Thomas, without exploring queer identities or experiences.

Peter Rabbit 2: The Runaway is a children's film centered on anthropomorphic animals and their adventures. It does not include any transsexual characters or themes, therefore, there is no portrayal to evaluate within its narrative.

The film is a family-friendly animated adventure. While female rabbit characters participate in various chases and mischievous capers alongside male characters, there are no instances where a female character defeats one or more male opponents in direct physical combat through skill, strength, or martial arts.

All established characters from Beatrix Potter's stories and the previous film maintain their canonical genders in "Peter Rabbit 2: The Runaway." No characters widely established as one gender are portrayed as a different gender.

The film features anthropomorphic animal characters and human characters whose race aligns with their established portrayals in the source material and prior film installments. No characters canonically established as one race are depicted as a different race.