The film 'Phineas and Ferb The Movie: Across the 2nd Dimension' does not include any identifiable LGBTQ+ characters or themes. Its narrative centers on family-friendly adventure and imagination, with no explicit or implicit representation of queer identities or relationships.

The film "Phineas and Ferb The Movie: Across the 2nd Dimension" does not feature any identifiable transsexual characters or themes. The narrative focuses on childhood inventions, alternate dimensions, and family dynamics, with no explicit representation or discussion of transgender identity.

The film features female characters like Alternate Candace and Alternate Isabella who participate in action sequences against robot forces. However, their victories are achieved through strategic planning, leading groups, or utilizing technology and vehicles, rather than direct close-quarters physical combat against male opponents.

The film is a direct continuation of the animated series, featuring the same established characters. All characters maintain their original, canonically established genders from the source material.

The film is an animated continuation of the 'Phineas and Ferb' television series, featuring the same established characters, designs, and voice actors. No characters who were canonically established as one race in the source material are portrayed as a different race in the movie.