The film centers on an intersex protagonist whose forced gender transition is integral to the plot. While the character experiences a tragic and paradoxical life, their unique identity is explored with dignity, complexity, and agency, affirming the worth of their non-normative existence within a complex sci-fi narrative.

Predestination features a central character whose physical sex changes due to a unique time-travel paradox, leading to a profound exploration of identity. This transformation is depicted with dignity and complexity, serving as a crucial plot point rather than a source of ridicule or negative portrayal. The character's journey, though tragic and paradoxical, is treated with respect, highlighting their agency and internal struggles, such as the protagonist's complex arc from Jane to John.