Religulous critically examines religious beliefs, including those that condemn homosexuality. By challenging the foundations of religious intolerance and highlighting extremist anti-LGBTQ+ views as irrational, the film implicitly supports LGBTQ+ dignity and rights, framing prejudice as an external force to be critiqued.

The film extensively critiques Christianity, highlighting perceived inconsistencies in its scriptures, the hypocrisy of some adherents, and its historical role in conflict and oppression, presenting its beliefs as irrational and dangerous.

The film heavily scrutinizes Islam, focusing on its fundamentalist interpretations, the concept of jihad, and its perceived intolerance, often drawing parallels with other Abrahamic religions regarding their potential for violence and irrationality.

Judaism is portrayed critically, particularly concerning its historical narratives, the concept of a chosen people, and its role in the Israeli-Palestinian conflict, often linking it to the broader critique of Abrahamic faiths.