The film uses its protagonist's extreme homophobia and the depiction of harmful stereotypes as a primary source of comedic shock value. While the intent may be satirical to expose bigotry, the direct portrayal of ridicule and degradation of queer identity, without an internal narrative counterbalance, results in a problematic net impact.

The film includes a brief, stereotypical depiction of a transvestite prostitute, primarily serving as an object for Borat's transphobic reactions and disgust. While intended to satirize bigotry, the portrayal itself lacks nuance or counter-critique, resulting in a net negative impact for transsexual themes.

The film satirizes certain aspects of American evangelical Christianity, portraying some adherents as prejudiced, easily manipulated, or hypocritical. While not a blanket condemnation, the narrative highlights problematic behaviors and beliefs within these interactions without a strong counterbalancing positive portrayal of the faith itself.

The film satirizes anti-Muslim prejudice by having Borat express bigoted views that are sometimes echoed by others, but the narrative unequivocally condemns such bigotry rather than the religion itself.

Borat's extreme antisemitism is consistently presented as ignorant and reprehensible, with the narrative clearly condemning his bigotry and positioning the audience to sympathize with Jewish individuals.