Resident Evil: Death Island does not feature any explicit LGBTQ+ characters or themes. The plot centers on action, horror, and a virus outbreak, with no narrative engagement with LGBTQ+ identities or issues. Consequently, the film offers no portrayal of the LGBTQ+ community.

Resident Evil: Death Island (2023) does not explicitly portray transsexual characters or themes. Based on available information, the film's narrative contains no prominent or detailed depictions of the trans community, leading to a classification of N/A for transsexual portrayal.

The film features Maria Gomez engaging in intense hand-to-hand combat with Leon S. Kennedy, a male opponent, but she is ultimately defeated. Other female characters like Jill and Claire participate in group combat against zombies, primarily using firearms, which does not meet the criteria for direct physical victories against male opponents.

All main characters in Resident Evil: Death Island, including Jill Valentine, Claire Redfield, Rebecca Chambers, Leon S. Kennedy, and Chris Redfield, retain their established canonical genders from the source material. There are no characters whose on-screen gender differs from their original portrayal.

The film is CG-animated, and character models maintain visual continuity with established designs from the source material. The voice actors' ethnicities align with the characters' established American or implied Hispanic origins. No character established as one race is portrayed as a different race.