Tokyo Gore Police portrays gender transformation and non-conformity primarily through extreme body horror and grotesque violence. Characters like the Key-Man, who undergoes a monstrous, involuntary gendered mutation, and 'Sister,' a male in drag depicted as a hunted 'freak,' contribute to a narrative where such identities are sources of degradation and fear, lacking any affirming or dignified representation.

The film features Ruka, a police officer, who consistently engages in and wins brutal close-quarters physical fights against multiple male opponents, including mutated 'Engineers' and other antagonists, using a katana and hand-to-hand combat.

The film's central protagonist is driven by a profound respect for her deceased father's legacy and a desire to avenge him, portraying a strong, foundational, and enduring parental bond that aligns with traditional values of filial piety and parental influence.