The film offers no depiction of LGBTQ+ characters or themes, focusing instead on a historical horror tale of Black soldiers confronting vampires in the Civil War era.

No transgender characters or themes are present in the film. The narrative centers on Black Union soldiers facing Confederate and supernatural threats in the Civil War era, offering no depiction of transsexual identity to assess.

The story centers on an all-male squad of Black Union soldiers infiltrating a Confederate fort, later aided by indigenous woman Tsula who provides navigational and supernatural knowledge. No female characters participate in or win direct physical combat against male opponents.

Resurrection Road presents an original narrative set during the Civil War, featuring newly created characters without adaptations from source material, prior films, or historical records that involve gender changes.

Resurrection Road introduces original characters in a Civil War-era horror narrative, with actors' races aligning with the depicted ethnicities of Union soldiers and Confederate figures, including historical guerrilla leader Quantrill portrayed by a white actor.