The film "Rooster Cogburn" does not feature any identifiable LGBTQ+ characters or themes. Its narrative is a traditional Western adventure focusing on a U.S. Marshal and a missionary, with no elements pertaining to queer identity.

The film "Rooster Cogburn" is a Western adventure that does not feature any identifiable transsexual characters or explore transgender themes. Its narrative focuses on the pursuit of outlaws by Rooster Cogburn and Eula Goodnight, making the concept of transsexual portrayal inapplicable to its content.

The film features Eula Goodnight as the primary female character. While she is present during action sequences and uses firearms for defense, there are no scenes depicting her defeating one or more male opponents in direct, close-quarters physical combat using skill, strength, or melee weapons.

The film features Rooster Cogburn, who retains his established male gender from the source novel and previous film. Other significant characters, such as Eula Goodnight, are original to this installment and do not represent a gender swap of a previously established character.

The film "Rooster Cogburn" (1975) features characters consistent with their established racial portrayals from the source novel and previous film adaptations. No character canonically, historically, or widely established as one race is portrayed on screen as a different race.