Scary Movie 2 utilizes effeminate stereotypes and implied same-sex encounters primarily for crude humor and ridicule. Characters are consistently mocked for their perceived sexuality or gender expression, and queer themes are exploited for shock and degradation. The film offers no positive counterbalance, reinforcing harmful stereotypes and presenting queer identity as a source of mockery.

Scary Movie 2 includes a scene where a character is tricked into intimacy with a male ghost disguised as a woman. The humor stems from the character's disgust upon this reveal, employing a 'deceptive' male-presenting-as-female trope. This portrayal, though not explicitly featuring a transsexual character, reinforces problematic stereotypes often associated with trans identity, resulting in a negative net impact.

The film portrays Christian (Catholic) exorcism rituals and its practitioners as absurd, ineffective, and undignified. The narrative uses the religious context for crude humor and sacrilege, offering no counterbalancing positive portrayal or nuance.