The film 'Spirit Riding Free: Spirit of Christmas' does not include any explicit LGBTQ+ characters or themes. The narrative focuses on the adventures of its main characters and their horses during the holiday season, without addressing queer identities or experiences.

The film "Spirit Riding Free: Spirit of Christmas" does not feature any identifiable transsexual characters or themes. Therefore, an evaluation of its portrayal of transsexual identity is not applicable.

The film focuses on adventure and friendship, primarily involving horses and their riders. There are no instances where a female character engages in or wins direct physical combat against one or more male opponents using hand-to-hand, martial arts, or melee weapons.

This film is a Christmas special for the animated series "Spirit Riding Free." All established characters, including Spirit and the main human protagonists, maintain their canonical genders from the series and its source material. No characters established as one gender in prior canon are portrayed as a different gender.

The film features characters original to the 'Spirit Riding Free' series. There are no instances where a character previously established as one race in source material or prior installments is portrayed as a different race.