The film does not feature any identifiable LGBTQ+ characters or themes. The narrative focuses on the crossover event between Batman and Superman, their respective villains, and heterosexual romantic subplots, without incorporating any queer representation or storylines.

The film does not feature any identifiable transgender characters or themes. Its narrative centers on the team-up between Batman and Superman to combat the combined threats of Lex Luthor and the Joker, without exploring any aspects of gender identity or transition.

The film features several female characters, including Lois Lane, Mercy Graves, Livewire, and Roxy Rocket. Mercy Graves engages in a brief physical confrontation with Batman but is quickly subdued. Livewire and Roxy Rocket primarily use superpowers or vehicles and explosives, respectively, and do not achieve victories in direct physical combat against male opponents. No female character is depicted defeating one or more male opponents in close-quarters physical combat.

All established legacy characters in the film maintain their canonical genders from prior source material and adaptations. No characters widely known as one gender are portrayed as a different gender.

All major and legacy characters, including Batman, Superman, Lex Luthor, and Lois Lane, are portrayed with the same racial depictions as established in their comic book source material and prior adaptations. No character canonically established as one race is depicted as a different race.

Not depicted in the film.