The film explores the platonic friendships and journey of a boy, a mole, a fox, and a horse, emphasizing universal themes of kindness, courage, and belonging. There are no identifiable LGBTQ+ characters or themes present in the narrative, resulting in an N/A classification for LGBTQ+ portrayal.

The film "The Boy, the Mole, the Fox and the Horse" does not feature any identifiable transsexual characters or themes. Its narrative focuses on the journey and friendship of a boy and three animals, exploring themes of kindness, courage, and self-acceptance without engaging with transgender identity.

This animated film features no significant female characters. The narrative focuses on themes of friendship and journey, and does not contain any combat or action sequences involving physical confrontation between characters.

The film is an adaptation of Charlie Mackesy's book, featuring the same four main characters: The Boy, The Mole, The Fox, and The Horse. All characters maintain their established male gender from the source material in the film adaptation.

The film's main characters are an unracialized boy and three animals. The source material, an illustrated book, does not establish a specific race for the boy, nor do the animal characters have a human race. Thus, no character's race was changed from a prior established depiction.