The film 'Harry Potter and the Order of the Phoenix' does not contain any explicit or implicitly depicted LGBTQ+ characters or themes. Therefore, its net impact on LGBTQ+ portrayal is N/A due to a complete absence of such content.

The film "Harry Potter and the Order of the Phoenix" does not feature any identifiable transsexual characters or themes. Its narrative focuses on magical conflict, coming-of-age, and political intrigue, without engaging with gender identity issues.

Female characters such as Hermione Granger, Ginny Weasley, Luna Lovegood, Nymphadora Tonks, Minerva McGonagall, and Bellatrix Lestrange participate in combat. However, all combat depicted for these characters is exclusively spell-based, not physical close-quarters combat.

The film adaptation faithfully portrays the genders of all established characters as they appear in J.K. Rowling's source novel. No characters canonically established as one gender are depicted as a different gender in the movie.

All major and supporting characters in "Harry Potter and the Order of the Phoenix" are portrayed by actors whose race aligns with their established depiction in the source material and previous film installments.