Harry Potter and the Half-Blood Prince does not feature any explicit LGBTQ+ characters or themes. The film's narrative focuses solely on heterosexual relationships and friendships, with no identifiable queer representation present within its storyline.

The film "Harry Potter and the Half-Blood Prince" does not feature any identifiable transsexual characters or themes. Its narrative is centered on the established magical world, its characters, and the ongoing conflict with Voldemort, without touching upon gender identity or transition.

The film features female characters participating in magical duels and conflicts. However, there are no scenes depicting a female character defeating one or more male opponents through direct physical combat, such as hand-to-hand, martial arts, or melee weapon fighting. Combat victories are achieved primarily through the use of magic.

All major and supporting characters in "Harry Potter and the Half-Blood Prince" (2009 film) maintain the same gender as established in J.K. Rowling's original book series. No characters canonically established as one gender are portrayed as a different gender in this adaptation.

A review of major and legacy characters in the film against their established portrayals in the source books and prior films reveals no instances where a character's race was changed from their canonical or widely recognized depiction.