No Time to Die features an incidental depiction of an LGBTQ+ character. Q's homosexuality is briefly acknowledged through a casual mention of a male date, but this detail is not central to the plot or his character arc. The portrayal is neither explored in depth nor used for positive or negative narrative impact, resulting in a neutral overall assessment.

The film features Paloma, a CIA agent, who demonstrates exceptional skill in close-quarters combat, defeating multiple male opponents using martial arts and physical techniques during a mission in Cuba.

The characters of Moneypenny and Felix Leiter, who were historically portrayed as white in previous installments and source material of the James Bond franchise, are portrayed by Black actors (Naomie Harris and Jeffrey Wright, respectively) in this film.