The Last GunFight, an action film focused on an assassin tournament, does not feature any identifiable LGBTQ+ characters or themes according to available sources. The narrative centers on action, survival, and revenge, with no noted representation or exploration of LGBTQ+ presence.

Due to a complete lack of information regarding the film 'The Last GunFight' and its plot, it is not possible to evaluate the portrayal of transsexual characters or themes. No identifiable depictions are present in the available data, leading to an N/A rating.

The film features female characters, including Charlotte Vega, who actively participate in a brutal competition involving various combat challenges. However, the provided information does not detail specific instances where a female character defeats one or more male opponents in direct physical combat.

The provided information indicates that 'The Last Gunfight' does not employ gender swapping. The film features original characters whose genders align with their actors, and there is no mention of source material or historical figures being adapted with altered genders.

The provided information indicates the film features a conventional international ensemble with ethnic diversity but explicitly states there is no indication of race swapping or reimagining characters with different racial identities.