The Mark of Zorro (1940) does not feature any identifiable LGBTQ+ characters or themes. The narrative is a traditional adventure romance, entirely focused on heterosexual relationships and societal conflicts of its historical setting, resulting in no LGBTQ+ portrayal.

The film "The Mark of Zorro" (1940) is a historical adventure film set in Spanish California. Its narrative focuses on swashbuckling action, romance, and political intrigue. There are no identifiable transsexual characters or themes present in the movie's plot or character arcs.

The film primarily focuses on the male protagonist, Zorro, and his sword fights against male antagonists and soldiers. Female characters, such as Lolita Quintero, are present but do not engage in or win direct physical combat against male opponents.

The 1940 film "The Mark of Zorro" adapts the established characters from Johnston McCulley's original story, including Zorro, his love interest, and supporting figures, without altering their canonical genders. All major roles maintain their gender as established in the source material.

The film adapts Johnston McCulley's novel, featuring characters of Spanish descent in early 19th-century California. All major characters, including Zorro, are portrayed by actors whose race aligns with their established canonical and historical depiction.