The film focuses on Ann Lee and the Shakers, a religious sect known for celibacy and communal living. Based on available information, there are no explicit LGBTQ+ characters, plotlines, or themes present in the narrative, which primarily explores historical and feminist aspects.

The available information for 'The Testament of Ann Lee, 2025' indicates no depiction of transsexual characters or themes. Plot descriptions, festival notes, and reviews consistently focus on Ann Lee and the Shakers, emphasizing religious, feminist, and utopian themes without any mention of transgender identities. For example, the film is described as a historical musical drama about Ann Lee and the Shakers, with no transgender attribution in cast or synopsis.

The movie does not contain any action or adventure elements.

The provided information explicitly states that no characters in the film have on-screen genders that differ from their established genders in historical records. All listed historical figures are portrayed by actors matching their historical gender.

The film depicts historical figures and communities from 18th-century England and colonial America, who were historically of European descent. All principal and supporting actors cast are also of European ancestry, aligning with the historical racial context.