The Three Caballeros is a 1944 animated musical that explores Latin American culture through the adventures of Donald Duck and his friends. The film does not include any identifiable LGBTQ+ characters or themes, focusing instead on cultural vignettes and platonic friendships.

The Three Caballeros is an animated musical film that does not include any discernible transsexual characters or themes. Its narrative focuses on cultural exploration and musical numbers, without engaging with gender identity topics.

The movie does not contain any action or adventure elements.

The film features established male characters Donald Duck and José Carioca, alongside new male character Panchito Pistoles. No characters canonically, historically, or widely established as one gender are portrayed as a different gender.

The film's main characters are anthropomorphic animals (Donald Duck, José Carioca, Panchito Pistoles) whose species and established cultural identities remain consistent. There are no human characters who were canonically established as one race and then portrayed as a different race in this film.