The film features an openly gay character, AJ, whose relationship is depicted as normal and loving. His sexual orientation is incidental to the main horror plot, neither uplifting nor denigrating his identity. While he meets a tragic end, it is part of the film's overall horror narrative, not a consequence of his identity.

Unfriended: Dark Web portrays a transsexual character whose identity is weaponized by antagonists for blackmail and psychological torture. Her deadname and pre-transition photos are used to inflict extreme distress and humiliation, framing her identity as a vulnerability. The narrative uses this exploitation as a horror device, leading to tragic outcomes without offering a counterbalancing positive affirmation.