The film does not feature any identifiable LGBTQ+ characters or themes. As a German Western from 1966, its narrative focuses on adventure and friendship in the American West, without engaging with queer identities or experiences.

Based on the provided movie details, there is insufficient information to determine if transsexual characters or themes are present in 'Winnetou and the Crossbreed, 1966'. Therefore, an assessment of its portrayal as positive, negative, or neutral cannot be made at this time.

The film features female characters, notably Apanatschi, who are central to the plot's conflict. However, these characters are primarily portrayed as being protected by the male protagonists and do not engage in or win direct physical combat against male opponents.

The film is an adaptation from the Winnetou series, featuring established characters like Winnetou and Old Surehand, who retain their canonical male genders. No significant character established as one gender in source material or prior installments is portrayed as a different gender.