Angie Tribeca features a recurring gay character, Dr. Scholls, whose eccentricities are a source of the show's absurd humor, but he is also a competent professional. Other instances, like an undercover plot involving a gay couple, are played for situational comedy. The portrayal is incidental, neither strongly affirming nor denigrating LGBTQ+ identity, aligning with the show's overall parody style.

The show features Detective Angie Tribeca, who frequently engages in and wins physical altercations against male suspects, subduing them through direct physical means during arrests and confrontations.