Berlin Station features a bisexual protagonist, Daniel Miller, whose relationships with both men and women are depicted with dignity and complexity. His sexual orientation is integrated naturally into his character and the narrative, avoiding stereotypes or negative framing. The portrayal is respectful and affirming, contributing positively to LGBTQ+ representation.

Berlin Station features Lena, a trans woman, as a complex and capable hacker. Her identity is handled respectfully, and she is depicted with dignity and agency, including a significant romantic relationship. The portrayal avoids harmful stereotypes, presenting her as an integral character whose trans identity is part of her whole self, contributing to a net positive impact.

The series frequently portrays extremist groups claiming Islamic motivations as primary antagonists, depicting their actions as violent and dangerous. While the show condemns extremism, the consistent association of Islam with these problematic elements, without significant counterbalancing positive portrayals, frames the religion negatively.