Brockmire features prominent gay characters, Charles and Beth, who are depicted with dignity, complexity, and agency. Their sexual orientations are integrated into their well-rounded personalities and storylines without being a source of mockery or inherent tragedy. The show's portrayal is affirming, treating their identities with respect within its comedic and dramatic framework.

Brockmire features Shirley, a trans woman, as a love interest for a main character. She is depicted as a successful, confident lawyer, with her trans identity handled respectfully. The show explores societal perceptions and personal hangups, ultimately affirming her dignity and the legitimacy of her relationships, contributing to a net positive portrayal.

The show frequently satirizes the hypocrisy and superficiality of characters who espouse Christian values, often contrasting their stated beliefs with their selfish or judgmental actions. The narrative uses Christianity as a backdrop for human foibles without offering significant counterbalancing positive portrayals of the faith itself.