Chicago P.D. features LGBTQ+ characters, including a main character's lesbian sister and her wife, whose relationship is consistently shown with normalcy and acceptance. Other storylines also handle queer identity respectfully, avoiding stereotypes. The show's overall portrayal is supportive and validating, integrating LGBTQ+ lives into the narrative without making identity a source of inherent conflict.

Chicago P.D. featured a storyline in Season 4 where a transgender woman was the victim of a hate crime. The episode handled the character's portrayal with dignity, emphasizing the tragedy of transphobic violence and the importance of seeking justice. The narrative condemned prejudice, affirming the worth of trans lives despite the tragic outcome.

Christianity is frequently depicted as a source of moral guidance, community support, or personal solace for various characters. While individual adherents may be flawed, the show's narrative generally affirms the positive aspects of the faith and its role in community life.

The show often features diverse communities and, when depicting Muslim characters or communities, tends to distinguish between the faith and extremist actions. The narrative implicitly or explicitly condemns Islamophobia, aligning with the definition of a positive portrayal where bigotry against the faith is challenged.

When Jewish characters or communities are featured, the portrayal is typically respectful, highlighting cultural traditions, community bonds, or individual faith. The narrative avoids negative stereotyping and does not present Judaism itself as problematic.