Craig of the Creek consistently portrays LGBTQ+ characters and relationships in a positive and affirming light. Queer identities are presented as normal aspects of diverse characters, contributing to an inclusive and validating environment without being the sole focus of their characterization or a source of conflict.

Craig of the Creek features the non-binary character Eliza, whose identity is affirmed through consistent use of they/them pronouns by other characters and a portrayal that emphasizes dignity, intelligence, and agency. The show integrates Eliza's identity naturally, contributing to an overall positive and validating representation.

The show features Kelsey Pokoly, a skilled warrior character who frequently engages in close-quarters combat. She is depicted using her sword to defeat multiple male opponents, such as the King's guards, in various battles and confrontations within the creek.