Crazy Ex-Girlfriend offers a largely positive and affirming portrayal of LGBTQ+ characters, primarily through Darryl Whitefeather's journey of self-discovery as a bisexual man. His relationship with White Josh and subsequent co-parenting are depicted with dignity, complexity, and humor, normalizing queer relationships and diverse family structures within the narrative.

Crazy Ex-Girlfriend features Maya, a recurring trans woman character, whose identity is present but not central to the narrative. She is depicted as an ordinary individual, contributing to a normalized and non-problematic portrayal without explicitly focusing on trans themes or arcs.

The show portrays Judaism and Jewish culture with significant depth, humor, and nuance through its protagonist, Rebecca Bunch, and her family. While satirizing cultural stereotypes, the narrative ultimately affirms Jewish identity and explores its complexities with empathy and understanding.