Critical Role consistently features a diverse array of LGBTQ+ characters across its campaigns. These characters are depicted with dignity, complexity, and agency, often engaging in central romantic relationships or having their identities respectfully integrated into their backstories. The show normalizes queer identities, treating them as integral parts of its world without resorting to harmful stereotypes or making identity a source of inherent misery.

Critical Role features transsexual characters, notably Dorian Storm, a trans man, whose identity is presented naturally and with dignity. The narrative portrays him as a complex individual with agency, focusing on his personal journey. Nothing negative is associated with his identity, contributing to an overall affirming depiction of trans lives.

The show features multiple female characters who engage in and win direct physical combat against male opponents. Beauregard Lionett, a monk, uses martial arts and a staff, while Yasha Nydoorin, a barbarian, uses a greatsword to defeat male adversaries in close-quarters encounters.