Designated Survivor features several LGBTQ+ characters in diverse roles, from family members to high-ranking government officials. Their identities are generally portrayed with dignity and respect, integrated naturally into the narrative. Challenges faced by these characters typically arise from external forces like political conspiracies or blackmail, rather than their queer identity being a source of inherent conflict or negativity within the show's perspective. The overall portrayal is affirming and inclusive.

The show features Dr. Andrea Frost, a transgender woman portrayed as a highly competent and respected scientist. Her trans identity is handled with dignity and normalization, not serving as a source of conflict or ridicule. The narrative treats her with respect, highlighting her professional contributions and agency. This depiction is affirming, presenting a positive and integrated portrayal of a transsexual character.

Christianity is often presented as a positive moral framework for characters, including the President, guiding their ethical decisions and providing comfort. The show generally portrays Christian faith and values with respect.

The show consistently distinguishes between extremist groups and the broader Islamic faith, portraying Muslim characters with loyalty and integrity. It actively condemns Islamophobia and highlights the prejudice faced by innocent Muslims due to the actions of terrorists.