Doom Patrol features prominent LGBTQ+ characters like Larry Trainor, whose journey from closeted struggles to self-acceptance is depicted with depth and dignity. Danny the Street/World, a genderqueer entity, serves as a powerful symbol of affirmation and a safe haven for diverse identities. The show consistently portrays LGBTQ+ lives with complexity and respect, framing their challenges as external societal issues rather than inherent flaws.

Doom Patrol positively portrays Danny the Street, a sentient, gender-fluid, non-binary character. Danny is depicted with dignity and agency, providing a safe haven for outcasts and fostering an affirming community. The show consistently maintains an empathetic and respectful stance towards gender-expansive identities, framing challenges as external prejudice rather than internal struggles.

The character Crazy Jane, consistently depicted as white in the source comics, is portrayed by a Latina actress in the show, constituting a race swap.