DOTA: Dragon's Blood features characters like Marci, whose deep bond with Mirana is widely interpreted as a central, loving, non-heteronormative relationship, depicted with dignity and emotional depth. Fymryn's implied non-binary identity is also presented respectfully. The series portrays these characters with complexity and agency, framing their challenges as external, thus creating a net positive and affirming impact.

The show features multiple female characters who engage in and win physical combat against male opponents. Fymryn uses daggers and agility, while Luna employs a glaive, demonstrating superior martial skill and strength in close-quarters encounters.

Several established characters from the Dota 2 video game, including Mirana, Luna, and Selemene, who were depicted with lighter skin tones in the source material, are portrayed with darker skin tones in the animated series.