Family Guy frequently features LGBTQ+ characters and themes, but its satirical and often offensive humor typically uses these identities as a source of mockery, discomfort, or stereotype. While some characters are eventually accepted, the comedic framing often relies on problematic tropes, leading to a net portrayal that endorses ridicule rather than consistent affirmation or dignity.

Family Guy features transsexual characters, most notably Ida Quagmire. While Ida is depicted with agency, the show's comedic approach frequently uses her identity as a source of shock humor and discomfort for other characters. The narrative often relies on stereotypes and makes trans identity the punchline, resulting in a net problematic portrayal.

The show frequently satirizes Christian hypocrisy, dogma, and the actions of its adherents, often portraying them as foolish, bigoted, or absurd. The narrative consistently uses Christianity as a target for comedic critique rather than affirming its virtues.

The show's portrayal of Islam often relies on problematic stereotypes, particularly concerning terrorism or extremism, for comedic effect. Its attempts at satire frequently blur the line between critiquing prejudice and reinforcing negative perceptions.

Judaism is frequently depicted through stereotypical characters, with humor often derived from perceived cultural traits or historical references. While sometimes satirizing ignorance, the portrayal often reinforces negative stereotypes without consistent narrative counterpoints.