Frieren: Beyond Journey's End does not feature identifiable LGBTQ+ characters or themes. The narrative focuses on universal themes like grief and legacy, with no central exploration of sexual orientation or gender identity issues, resulting in no depiction.

Frieren: Beyond Journey's End does not explicitly portray transsexual characters or themes within its narrative. While fan interpretations may suggest related concepts, the anime primarily focuses on fantasy and adventure, lacking any central or detailed exploration of trans identity or the trans community.

The series features powerful female mages like Frieren and Fern, who primarily engage in magical combat. While characters like Ubel use a knife, their victories are achieved through unique magical abilities (superpowers) rather than conventional physical combat, martial arts, or non-magically enhanced melee weapon skills.

The anime adaptation of "Frieren: Beyond Journey's End" faithfully portrays the genders of all established characters from the original manga. There are no instances where a character canonically established as one gender in the source material is depicted as a different gender on screen.

The anime adaptation of "Frieren: Beyond Journey's End" faithfully portrays its characters as depicted in the original manga. There are no instances where a character's established race or visual depiction from the source material has been altered in the on-screen adaptation.