Gintama frequently features LGBTQ+ coded characters and themes, including gender non-conformity and same-sex attractions. While some characters like Kyubei receive nuanced development, many portrayals lean on comedic exaggeration and stereotypes for humor. The overall impact is neutral, as the show neither consistently validates nor actively denigrates these identities, often using them as fodder for its irreverent comedy.

Gintama, 2006, frequently features gender-bending and cross-dressing for comedic purposes. These portrayals often rely on exaggerated stereotypes and derive humor from characters' discomfort or the absurdity of the situation, rather than exploring transsexual identity with dignity or complexity. The net impact is primarily negative, as identity is often a source of mockery, as seen in the 'Genderbend Arc' where gender swaps are played for laughs.

The anime features several prominent female characters who are highly skilled in physical combat. Kagura, Tsukuyo, and Kyubei Yagyu all repeatedly engage in and win close-quarters fights against multiple male opponents using martial arts, melee weapons, or superhuman strength.