Glee provided groundbreaking and largely affirming representation for LGBTQ+ characters on network television. Through central characters, it explored themes of coming out, love, acceptance, and overcoming prejudice with dignity and empathy. While not without minor flaws, the show's overall message strongly validated queer identities and relationships, making a significant positive impact.

Glee features multiple transgender characters whose journeys are depicted with empathy and support. Unique Adams's arc explores self-acceptance and overcoming prejudice, while Coach Beiste's transition is handled sensitively, emphasizing the importance of living authentically and receiving support from the community. The show's overall portrayal is affirming.

The show explores Christian faith with nuance, depicting characters' struggles and diverse interpretations. While critiquing hypocrisy or judgmentalism from some adherents, the narrative ultimately affirms the positive aspects of faith, such as hope, community, and comfort, aligning with core virtues.

Rachel Berry's Jewish identity is consistently portrayed with respect and is integrated as a positive and integral part of her character and family life. The show depicts her heritage and traditions without negative stereotypes.