Based on the limited information provided, an assessment of LGBTQ+ portrayal in 'Green Arrow, 2014' cannot be made. There is no available data to confirm the presence or nature of any LGBTQ+ characters or themes within the film.

There is insufficient information available to assess the portrayal of transsexual characters or themes in 'Green Arrow, 2014'. No plot details or character arcs related to transsexual identity were provided for analysis, making it impossible to determine any net impact.

The animated short film features established DC Comics characters like Green Arrow, Count Vertigo, and Princess Perdita, all of whom retain their canonical genders from the source material. No significant characters undergo a gender change.

The animated short features Green Arrow, Black Canary, and Count Vertigo. All characters are visually depicted on screen consistent with their established canonical races from the comics, despite one character being voiced by an actor of a different race.