Human Resources consistently features a diverse array of LGBTQ+ characters, including bisexual, gay, and non-binary individuals. Their identities are generally portrayed with dignity and complexity, integrated into the narrative as normal aspects of their personhood. Relationships are depicted as loving and valid, contributing to an overall affirming and inclusive representation.

Human Resources features Natalie, a trans woman, as a well-rounded character. Her trans identity is acknowledged and handled with respect, portraying her as a successful individual in a loving relationship. The show affirms her dignity and agency, presenting her identity positively without making it the sole focus of her character or a source of ridicule.