The film does not include any identifiable transsexual characters or themes. While the protagonist, Katie Mitchell, is depicted as queer (a cisgender girl who likes girls), her identity is not presented as transsexual. Therefore, the film has no depiction relevant to the evaluation criteria.

The film features female characters, such as Katie and Linda Mitchell, who participate in action sequences. However, their victories are primarily achieved against genderless robot antagonists, often through the use of technology, tools, or strategic thinking, rather than direct physical combat against male opponents.

The Mitchells vs. the Machines is an original film featuring characters created specifically for this production. There is no prior source material, historical basis, or previous installments from which characters' genders could have been established and subsequently altered.

The Mitchells vs. the Machines is an original animated film with characters created specifically for this production. There is no prior source material, historical record, or previous installment to establish a canonical race for any character that could then be altered.