Based on the provided information, there are no identifiable LGBTQ+ characters or themes present in 'In the Mud, 2025'. The plot and character descriptions focus on criminal activities, prison dynamics, and personal backstories without any mention of queer representation.

Currently, there is not enough information available to assess the film's portrayal of transsexual characters or themes. The provided search results lack specific details on this aspect, preventing a determination of any positive, negative, or neutral impact.

The movie does not contain any action or adventure elements.

The provided information explicitly states that no characters in "In the Mud" have on-screen genders that differ from their established genders in the source material, including those from its predecessor, *El Marginal*.

The series features one legacy character, Gladys Guerra, who is established as Argentine and portrayed by an Argentine actress. All other major characters are new to this spin-off, and their portrayals align with their described ethnicities/nationalities (e.g., a Spanish actress playing a Spanish character, a Colombian actress playing a Colombian character). No character's race was changed from prior canon or historical record.