Jane the Virgin features prominent LGBTQ+ characters, notably Luisa Alver and Petra Solano, whose same-sex relationships are integral to their character arcs. While Luisa's primary love interest is a villain, her identity is never denigrated. Petra's relationship with JR is depicted as deeply affirming and positive, showcasing growth and genuine love. The show consistently normalizes queer identities and relationships within its narrative.

"Jane the Virgin" features Krishna, a recurring trans woman character. Her identity is explicitly affirmed by a main character, Petra, who corrects a transphobic remark from her villainous mother. Krishna is portrayed with agency and complexity, and her trans identity is not a source of mockery or villainy, but rather a respectfully handled aspect of her character.

The show consistently portrays Catholicism as a source of moral guidance, community, and strength for its characters, particularly through Alba's devout faith and Jane's personal spiritual journey. While acknowledging modern challenges to traditional beliefs, the narrative ultimately affirms the positive role of faith in their lives.