Master of None offers a consistently positive and affirming portrayal of LGBTQ+ characters and themes. Through the character of Denise, the show explores queer identity, coming out, and marriage with dignity, complexity, and empathy, particularly highlighted in the acclaimed 'Thanksgiving' episode and the entirety of its third season.

Master of None features a transsexual character, Maya, in a segment of the episode 'New York, I Love You.' Her portrayal is incidental, depicting her daily life and dating experiences with dignity and without relying on harmful stereotypes. The show neither strongly uplifts nor denigrates her trans identity, presenting it as one aspect of a diverse New Yorker's life.

The show portrays Dev's parents' Hinduism with respect and warmth, highlighting it as a source of cultural identity and family values. While Dev, an atheist, navigates generational differences, the narrative consistently frames their faith as a positive and integral part of their lives, without mocking the religion itself.

The show portrays Islam positively through the respectful depiction of Denise's grandmother, a Black Muslim woman whose faith provided strength and community in the face of adversity and racism, particularly during the Civil Rights era.