Murdoch Mysteries consistently portrays LGBTQ+ characters with empathy and dignity, framing societal prejudice as the primary antagonist. While characters face historical challenges, the show's narrative affirms their worth and critiques the era's intolerance, resulting in a net positive and validating impact.

Murdoch Mysteries features a transgender character, Francesca, in an early 20th-century setting. The show portrays her with dignity and complexity. Despite initial societal confusion, the narrative, particularly through its lead characters, adopts a remarkably empathetic and respectful stance towards her identity, framing challenges as external prejudice.

The show consistently portrays Christianity, particularly Catholicism through Detective Murdoch, as a source of moral guidance, personal strength, and a framework for justice. While individual Christian characters may exhibit flaws, the narrative affirms the virtues and dignity of the faith, often contrasting bigotry against it with the inherent goodness of its adherents.

Judaism is consistently portrayed with respect, often highlighting the resilience and cultural richness of Jewish characters. The show's narrative explicitly condemns the antisemitism faced by Jewish individuals, positioning the audience to sympathize with the victimized religion and its adherents.