My Adventures with Superman features explicit and affirming LGBTQ+ representation through the queer couple Livewire and Heat Wave. Their relationship is depicted with emotional depth, on-screen affection, and is treated with dignity. Their story positively influences main characters, reinforcing themes of empathy and inclusivity, making the overall portrayal supportive and validating.

The show features Supergirl, a female character, who decisively defeats Superman, a male opponent, in direct physical combat. This confrontation highlights her superior combat skills and strength during their duel.

Lois Lane and Jimmy Olsen, characters traditionally established as Caucasian in their comic book origins, are portrayed as Korean and Black, respectively, in the series. This constitutes multiple instances of a race swap.