New Amsterdam consistently portrays LGBTQ+ characters, primarily Dr. Iggy Frome and Dr. Lauren Bloom, with dignity, complexity, and agency. Their queer identities are integrated naturally into their character arcs and relationships, which face typical human challenges rather than prejudice. The show's overall depiction is affirming, validating the worth of LGBTQ+ lives and love.

New Amsterdam features transsexual characters, including Dr. Leyla Shinwari and a patient named Kai. Leyla is portrayed as a skilled doctor and a complex individual, with her trans identity handled respectfully. Kai's storyline empathetically explores gender-affirming care. The show consistently depicts trans identities with dignity and affirmation, avoiding stereotypes or degradation.

The show consistently portrays Christian characters and their faith with respect, often highlighting how faith provides comfort, community, and guidance, even when navigating complex medical decisions. The narrative emphasizes empathy and understanding towards religious beliefs.

Muslim characters and their religious practices are presented respectfully, emphasizing their humanity, cultural identity, and the role of faith in their lives. The narrative often highlights the hospital's efforts to be inclusive and sensitive to Muslim patients' needs.

Jewish characters and traditions are depicted with nuance and respect, often showcasing the strength of community, cultural heritage, and personal faith. The show frequently accommodates Jewish practices within the hospital setting, affirming their importance.