One Piece features prominent LGBTQ+ characters like Ivankov, Bon Clay, and Kiku, who are depicted as powerful, brave, and loyal allies. Their identities are often central to their unique strengths and are consistently treated with respect and dignity by the narrative and their peers, contributing positively to themes of acceptance and self-worth.

One Piece features prominent gender non-conforming and transsexual characters like Emporio Ivankov, Mr. 2 Bon Clay, and Kikunojo. Despite some older 'okama' trope depictions having problematic comedic elements, these characters are consistently portrayed as powerful, brave, and loyal heroes. Their identities are often sources of strength and community, with Kikunojo receiving explicit affirmation. The net impact is largely positive, emphasizing their dignity and agency.

The show features multiple female characters who engage in and win close-quarters physical combat against male opponents. Characters like Nico Robin and Boa Hancock consistently demonstrate physical prowess and martial skill in defeating male adversaries through hand-to-hand techniques or melee attacks.