Rick and Morty features incidental LGBTQ+ representation, primarily through Rick Sanchez's implied pansexuality and his relationship with the gender-fluid hive mind, Unity. The show also includes alien species with diverse gender expressions. These elements are integrated into the narrative without strong positive affirmation or negative denigration, serving more as character details or sci-fi world-building.

Rick and Morty features a transsexual alien character, Kiara, whose identity is revealed as a comedic plot point. The narrative focuses on another character's discomfort rather than affirming or denigrating trans identity itself. Kiara is portrayed sympathetically, and the show's tone, through Rick's dialogue, suggests a dismissive acceptance of her identity.

The show features Summer Smith, who, with the aid of a cursed arm enhancing her physical abilities, engages in and wins direct physical confrontations against multiple male opponents.

The show frequently satirizes Christian themes and figures, often portraying religious belief as irrational, naive, or a source of conflict, aligning with Rick's cynical worldview.