Scream Queens features gay and bisexual characters, notably Boone Clemens, a gay villain, and Wes Gardner, who is revealed to be bisexual. The show's satirical and over-the-top nature means these identities are present and contribute to plot points, but are neither explicitly affirmed nor denigrated. The portrayal avoids strong positive or negative arcs, with queer identity being incidental to the show's broader dark comedy.

Scream Queens features Feather McCarthy, a transsexual character whose identity is primarily used for sensationalism and comedic shock value in a flashback plotline. Her portrayal lacks depth or dignity, serving as a plot device rather than a complex individual. A minor character also briefly mentions a 'transgender boyfriend' without further development, contributing to an overall problematic depiction.

The series consistently portrays characters who identify as Christian or make superficial religious references as deeply hypocritical, selfish, and morally bankrupt. The narrative uses these characters to satirize superficiality and self-interest, offering no significant counterbalancing positive or nuanced depiction of the faith or its genuine adherents.