No transgender characters or themes appear in the series. The story follows familiar young protagonists on supernatural adventures, with diversity limited to racial aspects among the ensemble.

Female characters including Eleven, Max, and Nikki participate in group confrontations against supernatural monsters and threats from the Upside Down. Eleven relies on telekinetic powers for victories. No scenes depict female characters defeating male human opponents through hand-to-hand combat, martial arts, or melee weapons.

Returning characters from the original Stranger Things series, including Eleven, Mike Wheeler, Dustin Henderson, Will Byers, Max Mayfield, and Lucas Sinclair, appear with their canonical genders. New characters such as Nikki Baxter, Anna Baxter, and Daniel Fischer are original creations without prior gender baselines, resulting in no gender swaps.

The series features the established Stranger Things characters in animated form without altering their canonical races, introducing new original characters instead.